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(A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with control or 10 mM NAM-supplemented buffer. <t>Electrocardiogram</t> <t>(ECG)</t> was recorded with the positive electrode (‘+’) positioned beneath the apex of the left ventricle, and the negative (‘-’) and ground (‘0’) electrodes on the cannula. Programmed electrical stimulation was applied via a pacing electrode (‘P’) placed on the surface of the right atrium. (B) Representative <t>ECG</t> traces from AF-negative (top) and AF-positive (bottom) mouse hearts. (C-E) AF incidence, episode count and burden in hearts from the control (n=6 for both sexes) and 10 mM NAM (n=5 for both sexes). Data are expressed as mean ± standard error of the mean. Chi-square test was used for AF incidence and Scheirer-Ray-Hare test followed by Dunn’s post-hoc test was used for AF episode count and burden.
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(A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with control or 10 mM NAM-supplemented buffer. <t>Electrocardiogram</t> <t>(ECG)</t> was recorded with the positive electrode (‘+’) positioned beneath the apex of the left ventricle, and the negative (‘-’) and ground (‘0’) electrodes on the cannula. Programmed electrical stimulation was applied via a pacing electrode (‘P’) placed on the surface of the right atrium. (B) Representative <t>ECG</t> traces from AF-negative (top) and AF-positive (bottom) mouse hearts. (C-E) AF incidence, episode count and burden in hearts from the control (n=6 for both sexes) and 10 mM NAM (n=5 for both sexes). Data are expressed as mean ± standard error of the mean. Chi-square test was used for AF incidence and Scheirer-Ray-Hare test followed by Dunn’s post-hoc test was used for AF episode count and burden.
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(A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with control or 10 mM NAM-supplemented buffer. <t>Electrocardiogram</t> <t>(ECG)</t> was recorded with the positive electrode (‘+’) positioned beneath the apex of the left ventricle, and the negative (‘-’) and ground (‘0’) electrodes on the cannula. Programmed electrical stimulation was applied via a pacing electrode (‘P’) placed on the surface of the right atrium. (B) Representative <t>ECG</t> traces from AF-negative (top) and AF-positive (bottom) mouse hearts. (C-E) AF incidence, episode count and burden in hearts from the control (n=6 for both sexes) and 10 mM NAM (n=5 for both sexes). Data are expressed as mean ± standard error of the mean. Chi-square test was used for AF incidence and Scheirer-Ray-Hare test followed by Dunn’s post-hoc test was used for AF episode count and burden.
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Figure 1. Phantom and <t>ECG</t> <t>Electrode</t> Affixing Diagram
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(A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with control or 10 mM NAM-supplemented buffer. Electrocardiogram (ECG) was recorded with the positive electrode (‘+’) positioned beneath the apex of the left ventricle, and the negative (‘-’) and ground (‘0’) electrodes on the cannula. Programmed electrical stimulation was applied via a pacing electrode (‘P’) placed on the surface of the right atrium. (B) Representative ECG traces from AF-negative (top) and AF-positive (bottom) mouse hearts. (C-E) AF incidence, episode count and burden in hearts from the control (n=6 for both sexes) and 10 mM NAM (n=5 for both sexes). Data are expressed as mean ± standard error of the mean. Chi-square test was used for AF incidence and Scheirer-Ray-Hare test followed by Dunn’s post-hoc test was used for AF episode count and burden.

Journal: bioRxiv

Article Title: Dual effects of nicotinamide on aging-related arrhythmia: protective at low dose, proarrhythmic at high doses

doi: 10.1101/2025.09.22.677785

Figure Lengend Snippet: (A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with control or 10 mM NAM-supplemented buffer. Electrocardiogram (ECG) was recorded with the positive electrode (‘+’) positioned beneath the apex of the left ventricle, and the negative (‘-’) and ground (‘0’) electrodes on the cannula. Programmed electrical stimulation was applied via a pacing electrode (‘P’) placed on the surface of the right atrium. (B) Representative ECG traces from AF-negative (top) and AF-positive (bottom) mouse hearts. (C-E) AF incidence, episode count and burden in hearts from the control (n=6 for both sexes) and 10 mM NAM (n=5 for both sexes). Data are expressed as mean ± standard error of the mean. Chi-square test was used for AF incidence and Scheirer-Ray-Hare test followed by Dunn’s post-hoc test was used for AF episode count and burden.

Article Snippet: Cardiac electrocardiograms (ECG) were recorded using three electrodes (MLA1213 and MLA1212, ADInstruments).

Techniques: Ex Vivo, Control

(A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with 0 mM NAM (control) or 100 mM NAM-supplemented buffer. (B) Representative electrocardiogram (ECG) shows that acute perfusion with 100 mM NAM rapidly induced VF, contractile dysfunction, and eventually led to cardiac arrest (n=4 males, 2 females). (C) Representiative blots and (D) quantification of acetylated SERCA2a levels of left venticular tissue from control and 100 mM NAM-perfused ex vivo mouse hearts (n=3). Data are expressed as mean ± standard error of the mean. Two tailed unpaired Student t-test was used.

Journal: bioRxiv

Article Title: Dual effects of nicotinamide on aging-related arrhythmia: protective at low dose, proarrhythmic at high doses

doi: 10.1101/2025.09.22.677785

Figure Lengend Snippet: (A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with 0 mM NAM (control) or 100 mM NAM-supplemented buffer. (B) Representative electrocardiogram (ECG) shows that acute perfusion with 100 mM NAM rapidly induced VF, contractile dysfunction, and eventually led to cardiac arrest (n=4 males, 2 females). (C) Representiative blots and (D) quantification of acetylated SERCA2a levels of left venticular tissue from control and 100 mM NAM-perfused ex vivo mouse hearts (n=3). Data are expressed as mean ± standard error of the mean. Two tailed unpaired Student t-test was used.

Article Snippet: Cardiac electrocardiograms (ECG) were recorded using three electrodes (MLA1213 and MLA1212, ADInstruments).

Techniques: Ex Vivo, Control, Two Tailed Test

Figure 1. Phantom and ECG Electrode Affixing Diagram

Journal: Polish Journal of Medical Physics and Engineering

Article Title: Investigation of the effectiveness of eight different metal artifact reduction algorithms in reducing extracorporeal metal artifacts: a phantom study using the Gumbel method

doi: 10.2478/pjmpe-2024-0023

Figure Lengend Snippet: Figure 1. Phantom and ECG Electrode Affixing Diagram

Article Snippet: Electrocardiogram (ECG) electrodes (Siemens Healthcare, Erlangen, Germany) were affixed to the trunk of a whole-body phantom (PBU-60; Kyoto Kagaku, Kyoto, Japan) as a source of metal artifacts for CT (Figure 1).

Techniques: